Metabolic Flux Analysis of Simultaneous Production of Vitamin B12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii
نویسندگان
چکیده
منابع مشابه
Propionic acid production in glycerol/glucose co-fermentation by Propionibacterium freudenreichii subsp. shermanii.
Propionibacterium freudenreichii subsp. shermanii can ferment glucose and glycerol to propionic acid with acetic and succinic acids as two by-products. Propionic acid production from glucose was relatively fast (0.19 g/Lh) but gave low product yield (~0.39 g/g) and selectivity (P/A: ~2.6; P/S: ~4.8). In contrast, glycerol with a more reduced state gave a high propionic acid yield (~0.65 g/g) an...
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Production of fermented functional foods containing micronutrients is required for their health beneficial properties. The impact of 11 process variables on vitamin B12 production in a dairy beverage containing propionic acid was investigated. Propionibacterium freudenreichii ssp. shermanii was applied in a 3-l fermentor in the fed-batch fermentation system. The most suitable conditions for...
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The in situ production of active vitamin B12 was investigated in aqueous cereal-based matrices with three strains of food-grade Propionibacterium freudenreichii. Matrices prepared from malted barley flour (33% w/v; BM), barley flour (6%; BF), and wheat aleurone (15%; AM) were fermented. The effect of cobalt and the lower ligand 5,6-dimethylbenzimidazole (DMBI) or its natural precursors (ribofla...
متن کاملStudy on solvent extraction of propionic acid from simulated discharged water in vitamin B12 production by anaerobic fermentation.
The potential of recovering propionic acid from discharged water in vitamin B(12) production by anaerobic fermentation was investigated in this paper. A primary amine, N(1923), was used as the extractant, kerosene as diluter and n-octanol as modifier. The influences of the content of N(1923) in the organic phase, the phase ratio and the pH of aqueous phase on the extraction yield of propionic a...
متن کاملBluB/CobT2 fusion enzyme activity reveals mechanisms responsible for production of active form of vitamin B12 by Propionibacterium freudenreichii
BACKGROUND Propionibacterium freudenreichii is a food grade bacterium that has gained attention as a producer of appreciable amounts of cobalamin, a cobamide with activity of vitamin B12. Production of active form of vitamin is a prerequisite for attempts to naturally fortify foods with B12 by microbial fermentation. Active vitamin B12 is distinguished from the pseudovitamin by the presence of ...
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ژورنال
عنوان ژورنال: Applied Biochemistry and Biotechnology
سال: 2021
ISSN: 0273-2289,1559-0291
DOI: 10.1007/s12010-021-03584-y